C++ library and its applications to generate and process accurate consensus sequences

• Getting Started
• Projects
  • Available
    • Consensus Core
    • Circular Consensus Calling ccs
    • Minor Variant Calling juliet
    • Reduce Alignment fuse
    • Swap Alignment Reference cleric
    • Minor Variant Pipeline julietflow
  • Planned
    • Genomic Consensus Calling gcpp
    • Viral Haplotype Phasing eden
• Developer environment
• PacBio open source license

ccs takes multiple reads of the same SMRTbell sequence and combines them, employing a statistical model, to produce one high quality consensus sequence.

gcpp will replace the current python GenomicConsensus, until then please use the existing solution.

juliet identifies minor variants from aligned ccs reads.

fuse reduces an alignment into its closest representative sequence.

cleric swaps the reference of an alignment by transitive alignment.

julietflow automatizes the minor variant pipeline.

eden will leverage CCS reads to identify low-frequency haplotypes within polyploid samples.

Support is only provided for official and stable SMRT Analysis builds provided by PacBio and not for source builds.