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RARD: Robust and accurate rare cell type de-convolution for gene expression and DNA methylation data

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Section 1: System Requirements

  RARD was implemented using python. It can be installed in Windows, Linux as well as MAC OS. RARD requires python version >= 3 and all the dependent packages will be installed using pip.

Section 2: Installation Instruction

  RARD can be installed using pip by the following command:

pip install RARD

Section 3: How to Use RARD

Section 3.1: Input Data Preparation

  We provide demon dataset generated from single cell mouse RNA-seq.

  Note: RARD is not sensitive to gene identifier, but the user should restrict gene identifier to a specific one.

Section 3.2: Deconvolution

We provide a demo here to illustrate how to run RARD

from RARD.run_deconRARD import deconvolution

deconvolution('ref.csv', 'mix.csv', 'marker.csv', 'prop_predict.csv', scale=0.01)

The results will be saved in prop_predict.csv.

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