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@UPSCb

Umeå Plant Science Centre Bioinformatics Facility

The UPSC bioinformatics facility

Umeå Plant Science Centre Bioinformatics Facility

Welcome to the GitHub organisation from the Umeå Plant Science Centre Bioinformatics Facility (UPSCb in short).

Repositories

Active

Repository Description DOI
course-web A repository to generate a simple website in nodejs to serve workshop content DOI
Dockerfile A collection of Dockerfile to create a variety of containers for bioinformatics analyses and workshops DOI
project-template A template to setup projects, based on the UPSCb guidelines for reproducibility not relevant
SeidRFile A repository containing an R package to interact with file in the seidr format (sf) generated by sei∂r DOI
UPSCb-common Common scripts and templates from the UPSCb. Cite it if you use it in your research. DOI

Tutorials

If possible, please cite the tutorials if you (re-)use them for your teaching. Mind the license and get in touch if you use these commercially.

Repository Description DOI
Tutorial_template A template to create learnR tutorials not-relevant
Tutorial_01_data_science_intro DOI
Tutorial_02_exploratory_data_analysis DOI
Tutorial_03_differential_gene_expression DOI
Tutorial_04_gene_set_enrichment_analysis DOI
Tutorial_05_ensemble_gene_set_enrichment_analysis DOI
Tutorial_06_differential_transcript_usage DOI

Published

Repository Description Manuscript DOI
aba Photoperiodic control of seasonal growth is mediated by ABA acting on cell-cell communication DOI
arabidopsis-floral-induction Characterisation of metabolic changes associated with floral transition in Arabidopsis: variations in raffinose synthesis contribute to the determination of flowering time. DOI
algalAcclimatization Algal acclimatization project
arabidopsis-aphids PECTIN ACETYLESTERASE9 affects the transcriptome and metabolome and delays aphid feeding
aspen-tension-wood-development-gradient A high spatial resolution transcriptome analysis across tension wood developmental gradient in Populus tremula reveals key players in biosynthesis of contractile G-layer TBA TBA
arabidopsis-nutrition-tor Arabidopsis-nutrition-tor
arabidopsis-prn2-hub2 Arabidopsis prn2 hub2 mutants analysis
aspen-seasonal-senescence aspen-seasonal-senescence
laccariaBicolorEcmDev The molecular timecourse of hybrid aspen/Laccaria bicolor ectomycorrhiza development
NorWood DOI
pine-needles-fungi T4F pine needles pacbio amplicon sequencing
poplar-CRISPR-WGS The splicing genes SmEa and SmEb regulate plant development during vegetative growth in poplar DOI DOI
poplar-early-bud-break Bud-break after winter dormancy is economically and environmentally important process in trees and shrubs from boreal and temperate latitudes but its molecular mechanisms are poorly understood. Previously we have reported the isolation and characterization of the EARLY BUD BREAK 1 (EBB1) gene, encoding an AP2/ERF transcription factor. We show here that EBB1 directly represses the poplar SVL (SHORT VEGETATIVE PHASE-LIKE), a MADS-box protein which was recently found to negatively regulate bud-break in Populus. We also report the identification and characterization of the EARLY BUD-BREAK 3 (EBB3) gene, which encodes another AP2/ERF transcription factor. We demonstrate that EBB3 is a positive regulator of bud-break and acts downstream of EBB1 and SVL. EBB3’s effect on bud-break is mediated via control of cell cycle by directly and positively regulating CYCLIND3.1 gene. Our results show the molecular architecture of a novel regulatory module that controls bud-break in poplar by controlling progression of cell cycle.
poplar-vegetative-growth-splicing
poplarCambium
PoplarFT FLOWERING LOCUS T Paralogs Control the Annual Growth Cycle in Populus Trees DOI DOI
riboSeqPipeline RiboSeq pipeline
SeedTransNet-seidr Seidr analysis for SeedTransNet
seidrSpruce Running seidr on spruce compendia
spruce-cone-setting Here, we utilize the early cone-setting acrocona mutant to study reproductive phase change in the conifer Picea abies; a representative of the gymnosperm lineage. The acrocona mutant frequently initiates cone-like structures, called transition shoots, in positions where wild-type P. abies always produces vegetative shoots. By sequence analysis of mRNA and microRNA transcripts, we demonstrate that a member of the SQUAMOSA BINDING PROTEIN-LIKE (SPL) gene family, PaSPL1, is active in reproductive meristems, whereas two putative negative regulators of PaSPL1, microRNA156 and the conifer specific miR529, both are upregulated in vegetative meristems and transition shoots. By allele-specific assembly, we identify a mutation in the miRNA binding-site of the acrocona PaSPL1 allele. Data presented suggest that the mutation renders the acrocona allele micro-RNA resistant. Finally, by genotyping a segregating population of inbred acrocona trees, we show a clear co-segregation between the early cone-setting phenotype and trees homozygous for the acrocona mutation.
spruce-pine-light Global gene expression analysis in etiolated and de-etiolated seedlings in conifers
spruce-seasonal-needles DOI
spruce-somatic-embryogenesis
spruce-wood-laser-microdissection Blokhina et al., Plant Physiology, 2019
UPSCb The original repository of the UPSC Bioinformatics Facility, holds multiple published projects, their analyses and links to their datasets TBA DOI
zygoticEmbryogenesis Spruce zygotic embryogenesis

Archive-able

Repository Description DOI
RnaSeqTutorial A long lived repository from an R package that was used for RNA-Seq workshops. DOI

Forks

Repository Description
MARVEL A fork of the MARVEL repository used during the initial phase of the spruce v2.0 genome sequencing project

Popular repositories Loading

  1. UPSCb-common UPSCb-common Public

    Common scripts

    HTML 15 13

  2. UPSCb UPSCb Public

    HTML 5 3

  3. Dockerfile Dockerfile Public

    Host the dockerfile from dockerhub/delhomme

    Python 2 2

  4. RnaSeqTutorial RnaSeqTutorial Public

    An R tutorial package that describes the pre-processing of RNA-Seq data - from the raw (FASTQ) data to the alignment (mapping) to a reference genome - and its subsequent differential expression ana…

    R 2 1

  5. project-template project-template Public template

    Shell 2 2

  6. aspen-seasonal-senescence aspen-seasonal-senescence Public

    aspen-seasonal-senescence

    HTML 1

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