The Role of Capillary Electromigration Separation Techniques in Bioanalysis

da Silva, José Alberto Fracassi; Carvalho, Alexandre Zatkovskis; Carrilho, Emanuel; de Jesus, Dosil Pereira; de Oliveira, Marcone Augusto Leal

doi:10.1007/978-3-030-82381-8_22

José Alberto Fracassi da Silva^5,6,
Alexandre Zatkovskis Carvalho⁷,
Emanuel Carrilho^6,8,
Dosil Pereira de Jesus^5,6 &
…
Marcone Augusto Leal de Oliveira^6,9

Abstract

Capillary electromigration techniques play a central role in bioanalysis. The full compatibility with aqueous solutions makes the electromigration techniques the best option for analyzing charged and polar compounds in complex matrices. Different mechanisms of separation (or modes of operation) using the same instrumentation but changing the separation medium can be applied to cover a wide range of chemical compounds. Indeed, electromigration techniques can handle from small ionic molecules to macromolecules and biopolymers. Additionally, sample preparation is simplified, and many online preconcentration techniques are available. Also, commercial instrumentation with a high degree of automation is available. This chapter discusses the instrumental aspects and the main modes of separations in capillary electrophoresis and addresses typical bioanalytical applications.

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Authors and Affiliations

Department of Analytical Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil
José Alberto Fracassi da Silva & Dosil Pereira de Jesus
National Institute of Science and Technology in Bioanalytics (INCTBio), Campinas, SP, Brazil
José Alberto Fracassi da Silva, Emanuel Carrilho, Dosil Pereira de Jesus & Marcone Augusto Leal de Oliveira
Center for Natural Sciences and Humanities (CCNH), Federal University of ABC (UFABC), Santo André, SP, Brazil
Alexandre Zatkovskis Carvalho
Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil
Emanuel Carrilho
Departamento de Química Analítica, Universidade Federal de Juiz de Fora, Instituto de Ciências Exatas, Juiz de Fora, Brazil
Marcone Augusto Leal de Oliveira

Authors

José Alberto Fracassi da Silva
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Alexandre Zatkovskis Carvalho
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Emanuel Carrilho
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Dosil Pereira de Jesus
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Marcone Augusto Leal de Oliveira
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Correspondence to José Alberto Fracassi da Silva .

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Editors and Affiliations

Institute of Chemistry, State University of Campinas, Campinas, Brazil
Lauro Tatsuo Kubota
Institute of Chemistry, State University of Campinas, Campinas, Brazil
José Alberto Fracassi da Silva
Institute of Chemistry, Federal University of Minas Gerais, Belo Horizonte, Brazil
Marcelo Martins Sena
Center for Natural and Human Sciences, Federal University of ABC, Santo Andre - SP, Brazil
Wendel Andrade Alves

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da Silva, J.A.F., Carvalho, A.Z., Carrilho, E., de Jesus, D.P., de Oliveira, M.A.L. (2022). The Role of Capillary Electromigration Separation Techniques in Bioanalysis. In: Kubota, L.T., da Silva, J.A.F., Sena, M.M., Alves, W.A. (eds) Tools and Trends in Bioanalytical Chemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-82381-8_22

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DOI: https://doi.org/10.1007/978-3-030-82381-8_22
Published: 26 November 2021
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-82380-1
Online ISBN: 978-3-030-82381-8
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)

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